Исита
liana.unitazによるHello, dear colleagues.
I present to you a study on:
"Evaluating the efficacy of injectable preparations in treating involutional changes of the face and neck, considering genetic markers and morphofunctional skin status."
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Scientists have proven that skin aging begins immediately after puberty. It becomes more noticeable between the ages of 35-50 and lasts until approximately 60 years of age. After that, a relatively stable period begins.
Skin aging is a complex, multicomponent, multifactorial, and prolonged process.
To date, there is no single definition of what skin aging is; however, it is already clear that it is caused by the accumulation of "defects" both in cells and in its intercellular structures.
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MAIN FACTORS OF AGING
First, hereditary (genetically) programmed changes affecting almost all layers of the skin;
Second, physiological (age-related) decline in hormone synthesis in the body. Hormones such as growth hormone, testosterone, DHEA (dehydroepiandrosterone), melatonin, etc., are essential for physical health and cell regeneration throughout the body, including the skin. Starting from the age of 20, the production of these hormones gradually decreases;
And third, photoaging – exposure to ultraviolet rays promotes the formation of free radicals, which negatively affect skin cell structures, significantly altering cellular repair mechanisms.
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Involutional skin changes, particularly of the face and neck, are of special relevance in this aspect, being one of the first easily identifiable visual signs of the body's overall aging.
Skin aging is a natural biological process occurring at the molecular, cellular, and tissue levels. The main manifestations of natural aging are thinning of the skin, increased skin texture and contrast pigmentation, moderate dryness, slight flaking, the presence of wrinkles acquiring characteristic forms for each age period.
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It is also important to note that the lifespan of extracellular matrix (ECM) proteins in general, and the system of elastic fibers in particular, determines the rate of molecular aging. Unlike intracellular proteins, whose half-life is measured in hours and days, the half-life of many extracellular proteins is on the order of several years. Therefore, to maintain youthful skin, it is necessary to protect its structural components and perform procedures that promote their active restoration.
Human genes cannot be changed. But by studying their characteristics, we can prevent and reduce the impact of negative factors on a specific individual. Knowing the weak points in gene function, we can direct efforts precisely there and thus influence the manifestation of certain external signs.
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In my work, I paid special attention to key genes that directly influence signs of skin aging.
These are genes responsible for the mechanical properties of the skin, as well as hydration and moisture loss.
According to the research results:
· The COL1A1 gene for detecting disorders in collagen structure – in most cases revealed impaired collagen fibril assembly.
· The ELN gene, which is responsible for skin elasticity, was prevalent in the majority of observed patients, indicating a reduced level of elastin in the skin and blood vessels.
· The AQP3 gene for hydration and moisture loss showed low levels of aquaporins in the subjects.
Research is also ongoing to study the correlation between genetic aberrations and various morphotypes of aging.
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For more detailed diagnostic data on the superficial and deep layers of the skin, a comprehensive assessment was performed using the Courage & Khazaka Intelligent Imaging System, which allows for a detailed assessment of skin sebum, porosity, fine superficial wrinkles, chromophores, and skin color. It also assesses deeper indicators such as pigment, vascular patterns (red areas), pathology of capillaries, and collagen status.
All these parameters were analyzed in accordance with the specific patient's genome.
Based on this, therapy was selected for the patient.
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Histological examinations of the skin from the area of interest were also conducted before and after therapy. This slide shows characteristic morphological involutional changes: inflammatory infiltration represented by lymphocytes and plasma cells with a slight increase in the number of mast cells; dilation of lymphatic vessels with edema of varying severity is observed.
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In an older patient, the skin thickness was greater, which can be associated with an increase in the amount of mature collagen in the reticular layer. Also, a difference in the degree of vessel dilation, especially lymphatic vessels, can be noted in the presented samples.
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The preparations we selected for therapy based on all the presented diagnostic patient data are skin bioreparants with a patented formula for zinc delivery into the cell and water-soluble silicon to activate high-quality neocollagenesis.
Thanks to the action of zinc, not only is the synthesis of new type 1 and type 3 collagen stimulated in the cell, but it also affects genetic stability by slowing down the process of telomere shortening, which leads to increased cell activity.
The developed zinc delivery formula is a system of gel globules of high molecular weight (1800 kDa) hyaluronic acid with inclusions of low molecular weight fragments (60-80 kDa) of a metal complex (Zn+HA).
Organic silicon, in turn, controls collagen formation at several "points": it stimulates mRNA synthesis, increases the enzymatic activity of prolyl hydroxylase, and promotes proline synthesis. This is necessary for the formation of the collagen triple helix, without which its full function is impossible.
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"Skinopro" preparations are a chelate complex in which the active components Zn(II)HA(20-60kDa) in the form of "hyasomes" (microscopic liquid-crystalline spherical, droplet-like particles) are uniformly distributed within the volume of high molecular weight HA (hydrogel).
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What functions do the components of the preparation perform?
Zinc: Is a cofactor for a large group of enzymes, including metalloproteinases involved in extracellular matrix remodeling.
· Has a proven positive effect on cellular aging biomarkers.
· A powerful antioxidant, as superoxide dismutase (which regulates lipid peroxidation) is a zinc-containing enzyme.
Silicon: Improves metabolic processes in cells.
· Stimulates collagen formation.
· Strengthens collagen structure (participates in the formation of the triple helix).
· Increases the physiological activity of fibroblasts.
· Regulates cellular metabolism.
· Reduces the development of fibrosis and lowers the level of glycation.
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In terms of clinical effects, I noted the correction of dehydration, improved skin tone and turgor, wrinkle correction, and correction of soft tissue ptosis.
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Protocols: Skinopro Active and Skinopro Forte.
We can work with these preparations using different techniques depending on the zone and indications for use.
We also actively treat the neck area, including the central and lateral surfaces.
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Patient: 49 years old
Diagnosis: Senile skin atrophy (L57.4)
Facial skin type: Combination
Morphotype: Tired; Fitzpatrick phototype: II; Glogau aging scale: Type 2
Gene COL1A1 showed moderate impairment of collagen fibril assembly.
Gene ELN indicates a reduced level of elastin in the skin and blood vessels.
Gene AQP3 showed a low level of aquaporins.
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Photos before therapy and 2 months after.
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We perform injections using the micro-papule technique across the entire face and neck area.
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Patient: 62 years old
Diagnosis: Skin changes caused by chronic exposure to non-ionizing radiation; Senile skin atrophy
Facial skin type: combination;
Morphotype: mixed;
Fitzpatrick phototype: II;
Glagau aging type 3
Genes: COL1A18%
ELN 39%
AQP3 38%
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And in the left lateral view...
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We can note significant improvements in skin quality due to skin flap retraction.
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And in the right lateral projection
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We see improvement in skin turgor, hydration and firmness.
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Next, I present the results of the histological study in the form of comparative diagrams, which clearly demonstrate the changes in tissue structure that led to the visible effect.
The research results show that in the skin of a 48-year-old patient after a course of "Skinopro Active" biorevitalizing procedures after 60 days, the level of type I collagen increased by 2.5 times, type III and IV collagen – by more than 3 times. In the samples of mature skin (62 years old) after completing therapy with "Skinopro Forte", type I collagen increased 2-fold, type IV collagen - 4-fold, and type III collagen almost 6-fold.
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A cumulative assessment of biomarker changes allows us to conclude that hydrogels with silicon ions have a positive effect on activating molecular processes that improve skin condition, significantly slowing down the aging process.
Moreover, in some cases, the combined presence of silicon and zinc ions leads to an even greater synergistic effect.
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And as Coco Chanel said:
"At twenty, we face the way nature intended; at thirty, the way we ourselves wish; but by fifty, we get the face we deserve."
